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Original Article
6 (
1
); 18-23
doi:
10.1055/s-0040-1712815

Comparative Study on Bone Mineral Densitometry in People Living with HIV with or without Highly Active Antiretroviral Therapy

Department of Medicine, Regional Institute of Medical Sciences, Imphal, India
Department of Medicine, Regional Institute of Medical Sciences, Imphal, India
Department of Radiodiagnosis, Shija Hospital, Imphal, India
Address for correspondence Ningthoukhongjam Reema, MBBS, MD, PGDGM, CCEBDM, Department of Medicine, Regional Institute of Medical Sciences, Lamphelpat, Imphal 795004, Manipur, India (e-mail: thangjamreema@gmail.com).
Licence
This open access article is licensed under Creative Commons Attribution 4.0 International (CC BY 4.0). http://creativecommons.org/licenses/by/4.0
Disclaimer:
This article was originally published by Thieme Medical and Scientific Publishers Private Ltd. and was migrated to Scientific Scholar after the change of Publisher.

Abstract

Introduction

Human immunodeficiency virus (HIV) is a disease that affects millions of people globally and affects almost all the body systems including bone metabolism. Derangement of bone mineral density (BMD) in HIV patients is well established in international literature but least studied in India. Therefore, this study aims to determine the association between BMD change and HIV infection with or without antiretroviral therapy (ART) and compare the different regimens of ART.

Materials and Methods

The cross-sectional study was conducted at the Department of Medicine and ART Center of Regional Institute of Medical Sciences, Imphal, India. A total of 50 HIV patients were screened by a central dual-energy X-ray absorptiometry (DEXA) examination for measuring BMD. Correlation of BMD with a CD4 count, and different ART regimens were also studied.

Results

In our study, majority of the patients (29 [58%]) had low BMD. Of the 29 patients, 18 (36%) had osteopenia and 11 (22%) had osteoporosis. Of the ART naïve patients, 81.8% have reduced BMD. Among different ART regimens, tenofovir-based regimes were mostly associated with low BMD (52.4%). A statistically significant association between low CD4 count and low BMD was found.

Conclusion

Our study concluded that HIV infection is associated with bone loss and low BMD in people living with HIV (PLHIV) irrespective of its treatment with ART. PLHIV are at a greater risk of bone loss secondary to decreased BMD. Among the ART regimens, tenofovir-based regimens are mostly associated with low BMD. Therefore, all HIV patients should be screened by DEXA scan for BMD status, and timely intervention should be started.

Keywords

bone mineral density
DEXA
HIV
osteoporosis

Introduction

Human immunodeficiency virus (HIV) is a disease that affects millions of people globally. In India, HIV prevalence is 0.22% (0.16–0.30) in 2017 in adult populations (age range: 15–49 years).1 Around 21.40 lakh people are living with HIV.1 Statewise, HIV incidence per 1,000 uninfected population in 2017 is highest in Mizoram (1.32) followed by Nagaland (0.59) and Manipur (0.58).1 Although Manipur has hardly 0.2% of India's population, it is contributing nearly 8% of India's total HIV positive cases (highest among the injecting intravenous drug users). HIV has emerged as a new and serious public health problem in Manipur. HIV infection virtually affects every system of the body, including hematologic system, central nervous system, respiratory system, cardiovascular system, and skeletal system. With the advent of highly active antiretroviral therapy (HAART), there has been a significant increase in the life expectancy of HIV infected patients; however, long-term effect and complications are also emerging, such as lipodystrophy, insulin resistance, diabetes, dyslipidemia, and alteration in phosphocalcic metabolism.2 Alterations in bone metabolism secondary to HIV infections, especially the change in bone mineral density (BMD) in these patients, are being researched with great interest. Low BMD has been reported in many cross-sectional studies involving younger3-8 and older9-12 HIV-infected individuals secondary to viral effects and inflammatory effects. Low BMD is significantly associated with a high risk of fracture in people living with HIV (PLHIV).13 Unfortunately, treatment with HAART also causes change in BMD. In one meta-analysis, the prevalence of osteoporosis was three times higher among HIV-infected patients than among HIV-negative controls, especially among those receiving antiretroviral therapy (ART).14 Among the ART, tenofovir (tenofovir disoproxil fumarate [TDF]) has proven to be the most widely involved agent in bone demineralization.15 Studies clarifying the cause–effect relationship underlying bone loss related to HIV infection are published from the developed world. So far, there are very few studies in India on this association, and no study has been conducted in this part of our country. Therefore, this study sought an accurate estimate of the prevalence of reduced BMD in a cohort of HIV-infected Manipuri patients with or without HAART. Its awareness could be harnessed to prevent the loss of bone density and subsequently negate potential fractures. This necessitated and provided us the impetus to perform this study to measure the change in BMD of HIV patients irrespective of its treatment with HAART.

Materials and Methods

This cross-sectional study was conducted at the Department of Medicine and ART Centre of Regional institute of Medical Sciences (RIMS), Imphal, India, for a period of 2 years (September 2016 to August 2018). BMD was measured in 50 HIV patients. In patients with low BMD, its correlation with CD4 count (HIV dependent factor) and body mass index ([BMI] HIV independent factor) were studied. This study also examined the change in BMD among the different regimens of HAART. They were divided into two groups: HAART therapy group and therapy-naive group. The therapy group was further divided according to the HAART regimen: first-line therapy group (tenofovir, lamivudine, and efavirenz [TLE]; zidovudine, lamivudine, and nevirapine [ZLN]) and second-line therapy group.

Inclusion Criteria

HIV-positive patients aged >18 years who visited the Medicine outpatient department or the ART Center, RIMS, or were admitted to the medicine ward were included in the study after obtaining informed consent.

Exclusion Criteria

Patients with other relevant comorbid conditions that can affect BMD were excluded from the study, such as bone disease, chronic metabolic diseases, chronic liver disease, chronic kidney disease, cancer, prolonged bedrest (more than 6 months), and patient on drugs such as corticosteroids, thyroxine, and lithium.

Personal details were recorded in proper performa, including age, sex, BMI, CD4 count, ART regime (first-line or alternative first- or second-line ART) and DEXA (dual-energy X-ray absorptiometry) report. A detailed relevant clinical examination of every patient was also performed. BMD was measured by DEXA scan using the lunar prodigy advance direct digital densitometry. The BMD was measured at the lumbar spine (L1-L4). Other relevant laboratory tests including hemoglobin, creatinine, liver enzymes, and albumin were also performed.

The World Health Organization (WHO) classifies BMD according to the number of standard deviations below the mean BMD for a healthy, young (25–35 years of age) sex- and ethnicity-matched reference population (T-score).16 For those aged > 50 years and postmenopausal women, a T-score of less than or equal to–2.5 at the hip or spine is defined as osteoporosis. Osteopenia is defined as a T-score between–1 and–2.49. A T score between 1 and–0.9 is normal. For those aged < 50 years, a Z-score of less than or equal to 2.0 is considered to be abnormal.17

HAART was defined as a combination of at least three antiretroviral agents that contained two nucleoside reverse transcriptase inhibitors (NRTIs) plus one protease inhibitor (PI) or one non-NRTI. Second-line ART should consist of a ritonavir-boosted PI plus two NRTIs, one of which should be zidovudine (azidothymidine) or TDF, based on what was used in the first-line therapy. Ritonavir-boosted atazanavir or lopinavir/ritonavir is the preferred PI.

Statistical analysis was performed using SPSS Version 20.0 (IBM Corp., Armonk, New York, United States). Mean and standard deviation were calculated for different study variables. Chi-square test and Student's t-test (two-tailed, independent) were used to test the association between BMD and HIV infection in PLHIV with selected variables of interest. A p-Value of <0.05 was considered as statistically significant.

Results

BMD was evaluated in a total of 50 HIV-infected patients whose baseline characteristics are shown in ►Table 1. The mean age of the patients in the study groups was 43.1 ± 7.2 years, with the majority aged 41 to 50 years (46%). Majority of the cases were females (72%), and males accounted for 28%. Females have lower bone mass with a faster rate of decreasing bone mass as compared with males.18 The mean BMI was 22 ± 4.1, and differences in BMI in the ART-naive group and the ART group was not statistically significant. Among 50 patients, 11 (22%) patients were ART-naive and 39 were on HAART. Based on different regimens, 19 (38%) patients were on TLE, 12 (24%) were on ZLN, and 8 (16%) were on second-line ART regimen. The mean BMD of PLHIV in our study was 1.05480. In our study, 21 (42%) patients had normal BMD on DEXA scan, whereas 29 had altered BMD changes (►Table 2). Of the 29 patients, 18 (36%) had osteopenia and 11 (22%) had osteoporosis. ART-naive patients had the lowest BMD among the groups. Among the patients taking ART (with different ART regimens), 52.4% of patients on TLE have low BMD. Patients on either ZLN or second-line ART had similar reduction in BMD (50%) (►Table 3). The difference in BMD in ART-naive patients and HIV patients on ART were statistically significant. Of the ART-naive patients, 81.8% have reduced BMD (either osteopenia or osteoporosis). Regarding the correlation of BMD with CD4 counts, PLHIV on HAART have a higher CD4 count than ART-naive HIV patients. Low CD4 count is associated with low BMD in HIV patients. The association between CD4 count and BMD is statistically significant (p < 0.05) (►Table 4).

Table 1 Summary of baseline characteristics of study patients
Characteristics Study patients (N = 50), n (%) Mean
Age (y)
    18–30 03 (06)
    31–40 17 (34)
    41–50 23 (46) 43.1
    51–60 07 (14)
Gender
    Female 36 (72)
    Male 14 (28)
BMI
    Not on ART 11 (22) 23.24
    On ART 39 (78) 22.55
    ART status (mean CD4 count)
    Not on ART 11 (22) 276.64
    On ART 39 (78) 582.38
ART duration (in years)
    Before initiation 11 (22) 0.981
    0–5 17 (34) 1.118
    5–10 12 (24) 1.050
    10–15 10 (20) 1.031
ART regimes
    ART naive 11 (22) 0.981
    TLE 21 (42) 1.057
    ZLN 10 (20) 1.059
    Second line 8 (16) 1.144
History of fracture
    ART naive 0
    On ART 0

Abbreviations: ART, antiretroviral therapy; BMI, body mass index; TLE, tenofovir, lamivudine, and efavirenz; ZLN, zidovudine, lamivudine, and nevirapine.

Table 2 BMD status on DEXA scans of HIV patients with and without ART
HIV patients BMD
Normal Reduced BMD (osteopenia/osteoporosis)
ART naive 02 (18.2%) 09 (81.8%)
ART regimens
TLE 10 (47.6%) 11 (52.4%)
ZLN 05 (50.0%) 05 (50.0%)
Second-line ART 04 (50.0%) 04 (50.0%)

Abbreviations: ART, antiretroviral therapy; BMD, bone mineral density; DEXA, dual-energy X-ray absorptiometry; HIV, human immunodeficiency virus; TLE, tenofovir, lamivudine, and efavirenz; ZLN, zidovudine, lamivudine, and nevirapine.

Note: It shows that 81.8% of ART-naive patients have reduced BMD (either osteopenia or osteoporosis). Among the different ART regimens, 52.4% of patients on TLE have low BMD. Patients on either ZLN or second-line ART had a similar reduction in BMD (50%).

Table 3 Mean BMD in ART-naive HIV patients and those receiving different ART regimens
ART regimens Mean BMD No. of patients
ART naive 0.98127 11
TLE 1.05695 21
ZLN 1.05970 10
Second line 1.14413 8
Total 1.05480 50

Abbreviations: ART, antiretroviral therapy; BMD, bone mineral density; HIV, human immunodeficiency virus; TLE, tenofovir, lamivudine, and efavirenz; ZLN, zidovudine, lamivudine, and nevirapine.

Note: Patients receiving TLE had the lowest BMD, whereas patients receiving second-line ART had the highest BMD among the groups. The difference in BMD in ART-naive patients and HIV patients on ART were statistically significant (p < 0.05).

Table 4 CD4 count in relation to BMD in HIV patients
DEXA scan Mean CD4 count, cells/mm3
Normal 674.81
Osteopenia/ osteoporosis 399.48

Abbreviations: BMD, bone mineral density; DEXA, dual-energy X-ray absorptiometry; HIV, human immunodeficiency virus.

Notes: It shows that a low CD4 count is associated with low BMD in HIV patients.

p-Value < 0.05 was statistically significant.

Discussion

Of the many diseases associated with HIV infections, change in bone metabolism is undermined and underdiagnosed. It may be because of the benign course of its natural history and less case fatality rate Altered bone metabolism and HIV infection of the bone lead to reduced bone size, mass, and strength in PLHIV.19 Of the many parameters of alteration in bone metabolism, we mainly focused on bone density with respective interpretation based on the standardized WHO BMD score and grading. Many international studies revealed changes in BMD in these people. Borderi et al and Duvivier et al found that HIV infection of osteoblasts is associated with a negative balance of bone remodeling.20,21 Decline in BMD following HIV, and its treatment leading to osteopenia and osteoporosis in both male and female patients with HIV22,23 as compared with non-HIV controls11,24 were reported. In our study, 29 (58%) patients had reduced BMD. A meta-analysis found that there was a 6.4-fold reduction in BMD value in HIV-positive patients.14

Understanding the pathogenesis of bone loss in HIV is as important as its aftereffects. HIV infection targets T cells, B cells, and monocytes, which modulate several cytokines, causing persistent systemic inflammation. Osteoclastogenic RANKL (receptor activator of nuclear factor-κB ligand) cytokine production is increased because of reaction with HIV glycoprotein (gp) 120 and stimulation by tumor necrosis factor (TNF) α whereas the bone-sparing osteoprotegerin (OPG) cytokine is decreased.25-27 These ultimately result in marked osteoclast and its precursors causing excessive bone resorption, bone loss, and decrease in BMD.28,29 RANKL/OPG markers were found to be positively associated with BMD and T-/Z-score in HIV-positive patients. Another cytokine interferon (IFN gamma) regulates the RANKL receptor through TNF receptor associated factor-6 [TRAF-6]) and leads to decreased osteoclast production.30,31 Decreased plasma OPG/RANKL ratio and a plasma RANKL concentration of >500 pg/mL in the HIV patients were associated with low BMD.28 Moreover, viral proteins also augments alteration in bone metabolism. HIV viral proteins Vpr (viral protein R) and gp120 stimulate osteoclast activity, whereas p55-gag suppresses osteoblast activity and increases osteoblast apoptosis. This bone loss may also be because of the functional regulation of osteoclasts due to higher levels of C-C chemokine receptor 5 (CCR 5)32

Osteoporosis was seen in 22% of cases in our study, which was similar to that of 23% by Bonjoch et al.33 The rate was much higher as compared with the study by Bruera et al.3 In western population, osteoporosis was found in approximately 10 to 13% of HIV-positive patients. An osteoporosis rate of 15% was reported by Brown and Qaqish.14 Approximately 3.7-fold higher occurrence of osteoporosis was recorded in PLHIV.14 The lowest osteoporotic rate encountered in HIV patients was 12%.34 The plausible theory for higher BMD in mainland Indian population could be because of late diagnosis with simultaneous multiple comorbid opportunistic infections. These variables may exaggerate BMD alteration. Ironically, in our state of Manipur, the population is less but with good literacy and education levels, leading to better societal awareness regarding HIV, and thereby PLHIV turnout and reach to medical facilities are much better. This makes early treatment possible with lesser late signs (early stage).

Having said that, still osteopenia was detected in 36% of the cases in our study, which was comparable with the study of Casado et al,35 who studied 285 patients and found the prevalence of osteopenia in HIV population to the tune of 36 to 57% depending on the presence of secondary factors. The rate of osteopenia detected by Brown and Qaqish was 67%,14 whereas Bonjoch et al reported a rate of 47.5%.33 A slightly similar value of 42% was reported by Sharma et al.34

Comparison-wise, a milder form of BMD loss, that is, osteopenia, was more common finding than osteoporosis.34 Osteopenia progresses to osteoporosis in approximately 12% of HIV patients.34 In our study too, osteopenia was more commonly detected than osteoporosis. Similar results were reported by Cazanave et al as well.22 They found that more than half the HIV-infected patients had osteopenia and one-third had more severe bone loss, that is, osteoporosis.22 McComsey et al also reported similar results.15 These findings suggest that although PLHIV patients were prone to bone mineral loss, the magnitude of loss is less and subjects to several variables.

HIV severity (with respect to CD4 count) also contributes to reduced BMD.36-38 Greater bone loss and BMD were found in HIV-infected individuals with a low CD4 cell count (<50 cells/mm3) than those with a higher CD4 cell count (>500 cells/mm3).39 In our study, we found that a reduction in BMD in HIV patients is associated with low CD4 count and is statistically significant. HIV patients with normal BMD in our study had a higher CD4 count (mean CD4 count: 674.81 cells/mm3) compared with patients having low BMD (mean CD4 count: 399.48 cells/mm3). Rivas et al also found a similar association between CD4 and BMD.23

Meanwhile, there were reports of fracture risk in PLHIV,13 with higher rates of approximately 30 to 70% than HIV-negative patients.15 In our study, fracture was not reported in any of the patients within the follow-up of 3 years possibly due to better health awareness.

Another important aspect was the treatment of HIV itself causing osteopenia and osteoporosis. All ART regimens at their initiation stages decrease BMD irrespective of the regimen. According to Knobel et al,6 both osteopenia and osteoporosis were reported in patients on HAART treatment and in therapy-naive patients, with a similar percentage of osteopenia and osteoporosis.

Among the patients on various HAART regimens in our study, 52.4% on TLE had low BMD. Patients on either ZLN or second-line ART had a similar reduction in BMD (50%). Specifically, tenofovir-based regimen causes more bone loss (1–3% greater bone mineral loss) as compare with others.40-42 BMD in the spine and hip were showing more decrease values with tenofovir than with other regimens.42 Similar findings were shared by Haskelberg et al as well.43 Biochemically, TDF is converted to tenofovir through di-ester hydrolysis in plasma. Selective uptake of tenofovir by osteoclasts causes cellular stress, which alters gene expression involved in signaling osteoblast activity, and this results in decreased bone formation.44 It also causes bone demineralization and loss of renal calcium and phosphate with compensatory reabsorption. Ironically, in our country, TDF-based regimen was often started as an initial first-line therapy for HIV treatment, unless contraindicated. For those patients on alternative first- or second-line ART having low BMD, TDF might have been used initially for a variable duration before switching to the latter. Therefore, contribution of TDF to low BMD, even in the aforementioned patients, was quite a possibility. Other ART drugs causing low BMD were PIs and NRTIs. Mechanism of PIs causes a reduction in the production of the active form of vitamin D 1,25(OH)2D by inhibiting 25-hydroxylase and 1-α hydroxylase, resulting in a decrease in calcium absorption and decrease in bone density. Also, PIs increase osteoclast activity through proteasomal degradation of TRAF- 6, leading to unblocking of RANKL signaling. PI also inhibits osteoblastogenesis. NRTIs, on the other hand, inhibit the DNA polymerase, thereby inhibiting the replication of mitochondrial DNA and causing mitochondrial damage. It leads to lactic academia and causes osteopenia. Acidosis is buffered by bone calcium hydroxyapatite loss, resulting in osteoporosis.15

It is worth mentioning that our study showed 81.8% of ART-naive patients having reduced BMD (either osteopenia or osteoporosis). This finding could be corroborated with that of the study by Kruger and Nell,45 who also suggested a high prevalence of osteopenia and osteoporosis in HAART-naive patients. Such findings could be explained by the fact that sustained HIV viremia (unchecked) results in chronic activation of affected host immune cells causing inflammation and bone remodeling. Also, viral proteins activation could result in similar mechanism cascade.45

Duration of such ART was also another important factor in reducing BMD. Decline in BMD was reported with prolonged continuous course of ART according to Grund et al,46 who evaluated 214 patients and observed that BMD in patients on continuous ART declined per year by 0.8% (hip), 0.4% (spine DEXA), and 2.4% (spine-quantitative CT [computed tomography]). Few studies reported a 2 to 6% drop in BMD within the initial 2 years after initiation of various ART regimens.15 However, in our study, the differences in BMD in relation to the duration of ART are not statistically significant.

Limitations

This study has some limitations. First, the small sample size of the study with no controls may influence the results. Second, the cross-sectional design of the study limits the extension of its interpretation to the causality of associations. Third, all the patients were from the same examination center and were not compared with ART patients from other centers or states, and therefore selection bias could not be excluded. Fourth, HIV viral load was not assessed because of limited resources. Despite these limitations, our study has the advantage of being the first study evaluating bone health in HIV patients in this area cohort.

Conclusion

We conclude that PLHIV are at a greater risk of bone loss. Among other factors, HIV itself and treatment with HAART (especially tenofovir) also contribute to reduction in BMD. Low BMI, advanced stage of disease, and low CD4 counts are associated with low bone mass in HIV-positive individuals. Low BMD increases the risk of potential fracture, thereby necessitating preventive measures. Further longitudinal studies involving a large population are required to obtain more conclusive results and to establish a causal association between HIV infection and bone loss. Therefore, we recommend screening of all HIV patients by DEXA scan for BMD status and timely intervention.

Conflict of Interest

None declared.

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